Part:BBa_K4858001:Design

F2RL3 LAMP NEB1 F3 Primer

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 4
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Design Considerations: Several factors guided the design of the F2RL3 LAMP NEB1 F3 Primer. The primer was tailored to bind exclusively to the F2RL3 gene's region with the PAR4 mutation, ensuring that amplification is specific and reduces off-target effects. Given the importance of the SNP in our assays, the primer was designed to ensure the SNP's effective amplification. Considerations were made to ensure the SNP position was adequately captured between the primer's binding sites.

Source

The sequence for our F2RL3 LAMP NEB1 F3 Primer has its origins in the genomic sequence of the F2RL3 gene. We began our design process by extracting the relevant F2RL3 sequence from the Benchling platform. After pinpointing the desired sequence segment, especially emphasizing the region around the SNP of interest, we obtained a physical version of the primer through custom DNA synthesis services (IDT).

References

1. Hyman, L. B., Christopher, C. R., & Romero, P. A. (2022). Competitive SNP-lamp probes for rapid and robust single-nucleotide polymorphism detection. Cell Reports Methods, 2(7), 100242. https://doi.org/10.1016/j.crmeth.2022.100242 </p>

2. Biolabs, N. E. (n.d.). NEB LAMP Primer Design Tool. NEB LAMP. https://lamp.neb.com/#!/